Most patient workups performed in the HLA Laboratory involve more than one test.  Please refer to the test profile description for sample requirements and test to be ordered.  For further assistance, please contact the HLA laboratory at (509) 232-4490.

HLA Class I (ABC) Typing
 HLA Class I (ABC) typings are performed by low-resolution sequence-specific primer (SSP) amplification.  This test can be performed using anticoagulated peripheral blood, lymph nodes or spleen.
HLA Class II (DRDQ) Typing 
 HLA Class II (DRDQ) typings are performed by low-resolution sequence-specific primer (SSP) amplification.  This test can be performed using anticoagulated peripheral blood, lymph nodes or spleen.
HLA B27 Typing 
 HLA B27 typings are performed low-resolution sequence-specific primer (SSP) low-resolution sequence-specific primer (SSP) amplification. This test can be performed usinganticoagulated peripheral blood samples.
Flow Cytometric Lymphocyte Crossmatch 
Patient serum is crossmatched with donor lymphocytes to detect donor specific antibodies in the patient serum.  Patient serum is also crossmatched against the patient’s lymphocytes to detect autoantibodies.  Antibody binding is visualized through fluorescent-labeled secondary antibodies on the flow cytometer.
Cytotoxic Lymphocyte Crossmatch
Patient serum is crossmatched with donor lymphocytes to detect donor specific antibodies in the patient serum.  T cell crossmatches are performed by antigloublin augmented complement dependent cytotoxicity and B cell crossmatches are performed by AMOS modified complement dependent cytotoxicity.
FlowPRA I/II Screen
 Patient serum is screened against a panel of beads coated with purified class I and class II HLA antigens and acquired by flow cytometry.  Results are expressed as the percent reactive antibody (PRA), which is the percentage of beads that reacted positively with the patient serum.  Antibody specificity cannot be defined by this test.
LABScreen Single Antigen Antibody Identification
 Patient serum is screened against an array of microbeads coated with purified HLA antigens. Antibodies that bind to the microbeads are labeled and detected using a fluorescent PE-conjugated anti-human IgG secondary antibody. LABScreen assays are acquired on the Luminex 200 benchtop fluorescence analyzer. LABScreen Single Antigen beads are used to determine Class I or Class II HLA antibody specificity. This is also referred to as Luminex Single Antigen antibody identification because of the instrument used to perform the assay.
HLA Matched Platelet Donor Search
For patients requiring HLA compatible platelets, an HLA donor search is performed in order to generate a list of compatible donors to be recruited.